Regulation of Human Eosinophil Precursor Production by Cytokines: A Comparison
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چکیده
The effect of a panel of recombinant human (rh) cytokines on the generation of human eosinophil precursors was assessed using a two-step culture technique. Normal human bone marrow was preincubated with different cytokine combinations in liquid culture before assessment of the number of eosinophil progenitors, which give rise to eosinophil colony-forming units (CFU-Eo) on secondary semi-solid culture with either interleukin-5 (IL-5). IL-3, or granulocyte-macrophage colony-stimulating factor. rhlL-3 or rhGM-CSF, but not rhlL-5. increased the number of CFU-Eo. CFU-Eo production by rhlL-3 or rhGMCSF was
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تاریخ انتشار 2003